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Design and Operation of the Trehalose Cycle in Yeast

The physiological hallmark of heat shock response in yeast is a rapid, enormous increase in the concentration of trehalose. Normally found in growing yeast cells and other organisms only as traces, trehalose becomes a crucial protector of proteins and membranes against a variety of stresses, including heat, cold, starvation, desiccation, osmotic or oxidative stress, and exposure to toxicants. Trehalose is produced from glucose 6-phosphate and uridine diphosphate glucose in a two-step process, and recycled to glucose by trehalases. Even though the trehalose cycle consists of only a few metabolites and enzymatic steps, its regulatory structure and operation are surprisingly complex. The goal of this project is to elucidate in fine detail the structure, regulation, and multi-level functioning of the pathway under normal and stress conditions.



References:

[1] Voit, E.O., and T. Radivoyevitch: Biochemical systems analysis of genome-wide expression data, Bioinformatics 16(11), 1023-1037, 2000.

[2] Voit, E.O.: Biochemical and genomic regulation of the trehalose cycle in Saccharomyces cerevisiae. J. Theor. Biol., 223, 55-78, 2003.

[3] Fonseca, L.L., C. Sánchez, H. Santos, and E.O. Voit: Complex coordination of multi-scale cellular responses to environmental stress. Mol. BioSyst. 7 (3), 731 - 741; DOI:10.1039/C0MB00102C, 2011.

[4] Ndukum, J. L.L. Fonseca, H. Santos, E.O. Voit, and S. Datta: Statistical inference methods for sparse biological time series data. BMC Syst. Biol. 5:57, 2011.

[5] Fonseca, L.L., P.W. Chen, and E.O. Voit: Canonical modeling of the multi-scale regulation of the heat stress response in yeast. Metabolites 2(1), 221-241, 2012.


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Biochemical and genomic regulation of the trehalose cycle in Saccharomyces cerevisiae Biochemical and genomic regulation of the trehalose cycle in Saccharomyces cerevisiae
from [2] from [2]